How is tissue sent for frozen section

A single copy of these materials may be reprinted for noncommercial personal use only. This content does not have an English version. This content does not have an Arabic version. See more conditions. Request Appointment. Observe the gross appearance of the tissue in terms of color, consistency, texture, and presence of sutures used to mark the anatomical position of the sample.

Identify the resection margins and planes. Use different ink colors for identification of medial and lateral margins. When cutting the tissue, ensure that the tissue is fresh, preferably dry, and free from any gauze, sutures, or staples.

The tissue is then cut into multiple small pieces to assist freezing. Multiple sections of the tissue should be obtained to help minimize error and understand the primary pathology. Use a sharp blade to cut the most crucial area using gentle pressure. To embed tissue in the mold, keep a small piece in the center of the mold and pour OCT in excess over it.

A tissue holder is then placed over the tissue. Place the tissue in the frozen section chamber. To hasten the process, cold spray can be used. Once the tissue is frozen, it will appear whitish. Place the in frozen tissue the holder of the microtome and trim to remove the excess OCT. This prepares the tissue surface for sectioning. Cut the tissue gently and spread it over the antiroll plate using a cooled brush.

Press a glass slide firmly over the section and fix immediately in methanol for 1 minute. Rapid fixation is a must as a delay results in swollen cells and hazy cell margins. The slide is first rinsed in tap water and put in hematoxylin for a minute. Place the tissue in a sterile container and immediately deliver to histology and hand off to histology staff for frozen section. Notify the Histology department ext. Muscle biopsies are received fresh.

A piece of gauze with a few drops of saline on the gauze can be added if the tissue is at risk to dry out. Nerve biopsies for biochemical studies should also be sent fresh to Histology. The biopsy is performed to determine suitability of the donor organ and evaluate for pre-existing disease.

A frozen section is required to evaluate the tissue in rapid fashion. Therefore, the tissue biopsy is submitted to the laboratory fresh, and rapid transport with appropriate communication is an absolute requirement to prevent tissue degradation. The transportation kit is delivered to the Pathology Lab and the department secretaries send the specimens to the reference lab. Refer to policy Lab Renal Biopsy Collection.

A separate piece lesional is needed for histology and is fixed in formalin. If able to deliver to laboratory immediately, send DIF specimen fresh or on saline-moistened gauze. Frozen section procedure. Advantages of frozen section biopsy. For more information. Inform yourself. Access your subscriptions. Access through your institution. Add or change institution. Free access to newly published articles. Purchase access. Rent article Rent this article from DeepDyve.

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