Why use borate buffer

NaOH boric acid deionised water Hazards: Caustic, can cause severe burns or damage eyes Possible acute or chronic health damage if inhaled or ingested None in the quanty used here Precautions: Use gloves, safety glasses and lab coat during preparation and use. Adjust the pH to 8 by adding boric acid or NaOH. Make up to 1 L with deionised water. Share, email or print. Sign up to join our network and access all content and features. If you already have an account, sign in , or connect with LinkedIn , Google.

Premium accounts now available! Sign up now and claim your premium account. Read more here. This site uses cookies. Some cookies are essential to make the site work and others help us improve our services. We have used alkaline-borate treatment to expand the capability of interphase FISH mapping. We show here that alkaline-borate treatment increases nuclear diameter, the interphase distance between probes on homologous chromosomes, and the distance between probes on the same chromosome.

We also show that the mean square distance between hybridization sites in borate-treated nuclei is proportional to genomic separation up to 4 Mb. Thus, alkaline-borate treatment enhances the capability of interphase FISH mapping by increasing the absolute distance between probes and extending the range of the simple relationship between interphase distance and genomic separation. Abstract Interphase fluorescence in situ hybridization FISH has been shown to be a means to map DNA sequences relative to each other in the kb to Mb genomic-separation range.

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Brand: Chem Lab CL Add to basket. Description Description A borate pH Buffer solution helps to maintain alkaline pH level via a boric acid-borate anion equilibrium. It is used in cell culture and analytical chemistry applications, including protein modification procedures. Physical Form. Boiling Point. For Use With.